期刊
BIOLOGY OF REPRODUCTION
卷 69, 期 2, 页码 475-482出版社
SOC STUDY REPRODUCTION
DOI: 10.1095/biolreprod.103.015867
关键词
sperm; sperm motility and transport; spermatid; spermatogenesis; testis
We isolated cDNA clones for the novel actin-like proteins T-ACTIN I and T-ACTIN 2, which are expressed specifically in the mouse testis. These clones were from a subtracted cDNA library that was enriched for haploid germ cell-specific cDNAs. The mRNA sizes and deduced molecular masses of t-actin 1/m-ACTl7b and t-actin 2/mACTl7a were 2.2 kilobases (kb) and 1.8 kb, and M-r 43.1 x 10(3) and M-r 47.2 x 10(3), respectively. The two deduced amino acid sequences had 60% homology, and they had approximately 40% homology with other actins. The T-ACTINs contained some of the conserved regions seen in other actins. Although the cellular locations of these two proteins are quite different (T-ACTIN-1 was found in the cytoplasm and T-ACTIN-2 was located in the nucleus), the expression of their proteins and mRNAs is controlled during development and limited during spermiogenesis. In contrast, only T-ACTIN-2 was present in sperm heads and tails. These results suggest that T-ACTINs play important roles in sperm function and in the specific morphogenesis of spermatozoa during spermiogenesis.
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