期刊
EXPERIMENTAL HEMATOLOGY
卷 31, 期 8, 页码 708-714出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0301-472X(03)00160-7
关键词
-
资金
- NHLBI NIH HHS [N01-HB-67141, 1 P01 HL67314-01A1, N01-HB-67138] Funding Source: Medline
Objective. Umbilical cord blood (UCB) T cells are predominantly CD45RA(+), secrete less cytokines, and have diminished cytotoxicity compared to adult peripheral blood (PB). We hypothesized that the functional impairment of bulk UCB cells results from the relative dominance of immature lymphocyte subsets. In this study we established the physiologic ranges of lymphocyte subsets in UCB, and contrasted those with adult PB. Materials and Methods. Four-color FACS was utilized to characterize surface and intracellular protein expression on lymphocyte subsets from fresh unmanipulated UCB and adult PB. Results. We found that UCB contain significantly higher absolute numbers of T cells, NK cells, and B cells than adult PB (p < 0.0001). UCB also contains more naive cells not only among CD4(+) and CD8(+) T cells but also among B lymphocytes (p = 0.003). Most UCB T cells are CD45RA(+)/CD62L(+) recent thymic emigrants with smaller TCRgammadelta (p < 0.0001) and CD25(+) subsets (p = 0.0068). Fewer UCB T cells display HLA-DR and CCR-5 activation markers (p < 0.0001) while the CD8(+)/CD57(+)/CD28(-) suppressor, CD8(+)/CD45RA(+)/CD27(-) cytotoxic, and skin homing CLA(+) T-cell subsets are absent altogether. Compared with adult PB, more cord blood T cells progress through cell cycle (p < 0.0001) and enter apoptosis (p = 0.0003). Unlike in adult PB, the majority of proliferating Ki-67(+) T cells in UCB retain a CD45RA(+)/RO-, CD69(-), CD25(-), HLA-DR- resting phenotype (p = 0.0002). Conclusion. Most T and B lymphocytes express a naive phenotype in cord blood while suppressor and cytotoxic T-cell subsets are absent. Cycling UCB T cells retain a naive immunophenotype that may represent homeostatic expansion rather than antigen-driven proliferation. (C) 2003 International Society for Experimental Hematology. Published by Elsevier Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据