期刊
ORAL MICROBIOLOGY AND IMMUNOLOGY
卷 18, 期 4, 页码 203-207出版社
WILEY
DOI: 10.1034/j.1399-302X.2003.00043.x
关键词
bacterial adherence; in vitro pellicle; in vivo pellicle; periodontopathogenic bacteria; plasma proteins
Previous studies on dental pellicle formation and bacterial adherence have focussed on saliva and its components. The tooth surface is, however, also exposed to the plasma-derived crevicular fluid. In the present study, (i) plasma proteins in in vitro and in vivo pellicles were examined using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and image analysis and (ii) the adherence of periodontopathogenic bacteria to experimental plasma and saliva pellicles was examined using radio-labelled bacteria and liquid scintillation counting. The plasma components fibrinogen, fibronectin, albumin and IgG were incorporated from plasma in the experimental pellicle and mediated the adherence of Porphyromonas gingivalis , Fusobacterium nucleatum and Actinomyces naeslundii. These proteins were also readily detected in in vivo pellicles and were found to a higher extent in pellicles formed at the gingival part of the tooth surface than at the incisal part.
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