期刊
CLINICA CHIMICA ACTA
卷 334, 期 1-2, 页码 87-94出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0009-8981(03)00191-8
关键词
8-hydroxyguanine; competitive ELISA; oxidative stress; bladder cancer; prostate cancer
Background: 8-hydroxydeoxyguanosine (8-OHdG) is the most frequently detected and studied DNA lesion. Upon DNA repair. 8-OHdG is excreted in the urine. Urinary 8-OHdG is now considered as a biomarker of generalized, cellular oxidative stress and is linked to degenerative diseases including cancer. Methods: We developed a competitive enzyme-linked immunosorbent assay (ELISA) for urinary 8-OHdG by coating BSA conjugated 8-hydroxyguanine (8-OHG) on a microplate. Urine specimens containing 8-OHdG and monoclonal anti-8-OHdG antibody were incubated together in the microwell. Final quantification of bound anti-8-OHdG antibody was estimated by the addition of HRP-conjugated sheep-anti-mouse antibody. Results: The concentration range of the calibration curve was 0-60 ng/ml. The sensitivity of the assay was 0.5 ng/ml. The within-day precision and day-to-day precision were < 10%. The ELISA correlated well with a commercial kit (r=0.9). Our assay measured not only 8-OHdG but also 8-OHG and 8-hyroxyguanine in urine. Increased urinary concentration of 8-OHdG and its analogs were detected in both patients with bladder cancer (70.5 +/- 38.2 ng/mg creatinine) and prostate cancer (58.8 +/- 43.4 ng/mg creatinine) as compared to the healthy control (36.1 +/- 24.5 ng/mg creatinine). Conclusion: Our preliminary data suggest that the competitive ELISA for 8-OHdG and its analogs appears to be a simple method for quantifying the extent of oxidative stress and may have potential for identifying cancer risk. (C) 2003 Elsevier Science B.V. All rights reserved.
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