期刊
JOURNAL OF CELL BIOLOGY
卷 162, 期 3, 页码 489-498出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303039
关键词
glial cells; nodes of Ranvier; myelin sheath; growth cones; microvilli
类别
资金
- NCRR NIH HHS [1S10RR15775] Funding Source: Medline
- NINDS NIH HHS [5T32NS07366, T32 NS007366, R01 NS036637, R01NS36637] Funding Source: Medline
Nodes of Ranvier are specialized, highly polarized axonal domains crucial to the propagation of saltatory action potentials. In the peripheral nervous system, axo-glial cell contacts have been implicated in Schwann cell (SC) differentiation and formation of the nodes of Ranvier. SC microvilli establish axonal contact at mature nodes, and their components have been observed to localize early to sites of developing nodes. However, a role for these contacts in node formation remains controversial. Using a myelinating explant culture system, we have observed that SCs reorganize and polarize microvillar components, such as the ezrin-binding phosphoprotein 50 kD/regulatory cofactor of the sodium-hydrogen exchanger isoform 3 (NHERF-1), actin, and the activated ezrin, radixin, and moesin family proteins before myelination in response to inductive signals. These components are targeted to the SC distal tips where live cell imaging reveals novel, dynamic growth cone-like behavior. Furthermore, localized activation of the Rho signaling pathway at SC tips gives rise to these microvillar component-enriched caps and influences the efficiency of node formation.
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