期刊
NEUROREPORT
卷 14, 期 11, 页码 1481-1483出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200308060-00015
关键词
Ca imaging; neurotrophin; PLC-gamma; pyramidal neuron; TrkB receptor tyrosine kinase
A 1-2 min application of brain-derived neurotrophic factor (BDNF; 20 ng/ml) induced sustained elevation of intracellular Ca2+ lasting > 90 min, using the fura-2 imaging of intracellular Ca2+ mobilization, in visual cortical pyramidal neurons isolated from rats. BDNF increased intracellular Ca2+ through the PLC-gamma phosphorylation after the TrkB receptor tyrosine kinase activation. Either K252a or U73122 suppressed intracellular Ca2+ in the absence of BDNF We suggest that sustained activation of Trk B receptor tyrosine kinase and PLC-gamma occurs after a brief BDNF application and contributes to the short-term maintenance (< 30 min) of the sustained intracellular Ca2+ elevation.
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