期刊
NEUROREPORT
卷 14, 期 11, 页码 1485-1488出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/00001756-200308060-00016
关键词
excitatory postsynaptic currents; GluR2 AMPA receptor; inhibitory postsynaptic currents; medial vestibular nucleus; neonatal rat
Spontaneous synaptic currents of medial vestibular nucleus (MVN) neurons were studied using whole cell recording in slices prepared from 13- to 17-day-old rats. The spontaneous inhibitory postsynaptic currents (sIPSCs) were significantly reduced by the GABA(A) antagonist bicuculline (20 muM), but were not affected by the glycine antagonist strychnine (1 muM). The frequency, amplitude, and decay time constant of sIPSCs were 4.3 +/- 0.9 Hz, 18.1 +/- 2.0 pA and 8.9 +/- 0.4 ms, respectively. The specific AMPA receptor antagonist GYKI-52466 (50 muM) completely blocked the non-NMDA-mediated spontaneous excitatory postsynaptic currents (sEPSCs), indicating that they are mediated by an AMPA-preferring receptor. The AMPA-mediated sEPSCs were characterized by low frequency (1.5 +/- 0.4 Hz), small amplitude (13.9 +/- 1.9 pA) and rapid decay kinetics (2.8 +/- 0.2 ms). The majority (15/21) displayed linear I-V relationships, suggesting the presence of GluR2-containing AMPA receptors.
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