期刊
NEUROSCIENCE LETTERS
卷 346, 期 3, 页码 182-186出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0304-3940(03)00571-8
关键词
hypocretin/orexin; kinetics; calcium influx; cerebrospinal fluid
Different potencies for hypocretin-1 and -2 in sleep-wake regulation and feeding after intracerebroventricular (ICV) administration have been reported. These differences were often explained by the selectivity of the two hypocretins for hypocretin receptor-1 and -2, but little attention has been paid to kinetics of hypocretin peptides. We investigated the kinetics of the ICV hypocretin-1 and -2 in rats. ICV hypocretin-1 (10 nmol) increased hypocretin-1 peptide level in the CSF by 800-fold from baseline with the elevation lasting over 4 h. In contrast, after ICV hypocretin-2 (10 nmol), no significant rise in the CSF was found. CSF hypocretin levels were significantly correlated with the biological activities of CSF hypocretin-1 and -2 using the Ca2+ mobilization assay. Difference in the kinetics of hypocretins should be considered for interpreting ICV effects of hypocretins. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据