期刊
ENZYME AND MICROBIAL TECHNOLOGY
卷 33, 期 2-3, 页码 206-211出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0141-0229(03)00119-4
关键词
Rhodotorula bacarum; exopolysaccharide; pullulan production; BIOLOG
Yeast strain Y68, producing a large amount of pullulan, was isolated from the leaves collected in the south of China. This strain was identified to be Rhodotorula bacarum by BIOLOG analysis and routine yeast identification. This is the first time to report that pullulan was produced by R. bacarum. The optimal medium for pullulan production by this strain was 8.0% (w/v) glucose, 2.0% (w/v) soybean cake hydrolysate, 0.5% (w/v) K2HPO4, 0.1% (w/v) NaCl, 0.02% (w/v) MgSO4.7H(2)O, 0.06% (w/v) (NH4)(2)SO4, pH 7.0. The optimal cultivation conditions for pullulan production by this strain in 300-ml shake flask containing 50 ml of medium were observed at 28 C and with 180 rpm. Under these conditions, 5.9% (w/v) pullulan was produced within 60 h. This was the highest pullulan yield produced by yeasts obtained so far. No pigment in the medium was observed during the fermentation, suggesting that strain Y68 was a non-pigmented yeast strain. (C) 2003 Elsevier Science Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据