4.8 Article

Comparative analyses of multi-species sequences from targeted genomic regions

期刊

NATURE
卷 424, 期 6950, 页码 788-793

出版社

NATURE PUBLISHING GROUP
DOI: 10.1038/nature01858

关键词

-

向作者/读者索取更多资源

The systematic comparison of genomic sequences from different organisms represents a central focus of contemporary genome analysis. Comparative analyses of vertebrate sequences can identify coding(1-6) and conserved non-coding(4,6,7) regions, including regulatory elements(8-10), and provide insight into the forces that have rendered modern-day genomes(6). As a complement to whole-genome sequencing efforts(3,5, 6), we are sequencing and comparing targeted genomic regions in multiple, evolutionarily diverse vertebrates. Here we report the generation and analysis of over 12 megabases (Mb) of sequence from 12 species, all derived from the genomic region orthologous to a segment of about 1.8 Mb on human chromosome 7 containing ten genes, including the gene mutated in cystic fibrosis. These sequences show conservation reflecting both functional constraints and the neutral mutational events that shaped this genomic region. In particular, we identify substantial numbers of conserved non- coding segments beyond those previously identified experimentally, most of which are not detectable by pair-wise sequence comparisons alone. Analysis of transposable element insertions highlights the variation in genome dynamics among these species and confirms the placement of rodents as a sister group to the primates.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.8
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据