期刊
JOURNAL OF CHROMATOGRAPHY A
卷 1009, 期 1-2, 页码 197-205出版社
ELSEVIER
DOI: 10.1016/S0021-9673(03)01034-3
关键词
proteomics; Escherichia coli; two-dimensional liquid chromatography; proteins
This work demonstrates the development of a method for the analysis of complex proteome samples by two-dimensional nano-liquid chromatography-mass spectrometry. This approach includes strong cation-exchange, sample enrichment, reversed-phase chromatography and nanospray ion trap mass spectroscopy with data dependent tandem mass spectrometry spectra acquisition, and subsequent database search. The new methodology was first evaluated using standard protein digest samples. Finally, data for the analysis of a total Escherichia coli proteome are provided. (C) 2003 Elsevier B.V. All rights reserved.
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