期刊
JOURNAL OF CELL BIOLOGY
卷 162, 期 4, 页码 551-563出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303167
关键词
kinetochore; mitosis; cell cycle; LENP-E; BubR1
类别
资金
- NCI NIH HHS [T32 CA067754, T32 CA 67754] Funding Source: Medline
- NIGMS NIH HHS [R37 GM029513, R37 GM 25913] Funding Source: Medline
Centromere-associated protein-E (CENP-E) is an essential mitotic kinesin that is required for efficient, stable microtubule capture at kinetochores. It also directly binds to BubR1, a kinetochore-associated kinase implicated in the mitotic checkpoint, the major cell cycle control pathway in which unattached kinetochores prevent anaphase onset. Here, we show that single unattached kinetochores depleted of CENP-E cannot block entry into anaphase, resulting in aneuploidy in 25% of divisions in primary mouse fibroblasts in vitro and in 95% of regenerating hepatocytes in vivo. Without CENP-E, diminished levels of BubR1 are recruited to kinetochores and BubR1 kinase activity remains at basal levels. CENP-E binds to and directly stimulates the kinase activity of purified BubR1 in vitro. Thus, CENP-E is required for enhancing recruitment of its binding partner BubR1 to each unattached kinetochore and for stimulating BubR1 kinase activity, implicating it as an essential amplifier of a basal mitotic checkpoint signal.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据