期刊
FEBS LETTERS
卷 550, 期 1-3, 页码 149-154出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00757-9
关键词
ethylene response factor; 3D modeling; lycopersicon esculentum
Four new members of the ERF (ethylene-response factor) family of plant-specific DNA-binding (GCC box) factors were isolated from tomato fruit (LeERF1-4). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N-terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three-dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis-element sequence and the nature of critical amino acid residues within the ERF domain. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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