期刊
JOURNAL OF CONTROLLED RELEASE
卷 91, 期 1-2, 页码 53-59出版社
ELSEVIER
DOI: 10.1016/S0168-3659(03)00213-X
关键词
water soluble polymers; tat peptide; confocal microscopy; subcellular fractionation
资金
- NCI NIH HHS [CA51578] Funding Source: Medline
One of the main obstacles to efficient intracellular delivery of therapeutic macromolecules is the barrier posed by the plasma membrane. In this study, the cell penetrating peptide Tat was conjugated to a synthetic macromolecule based on N-(2-hydroxypropyl)methacrylamide (HPMA) and its subcellular distribution in human ovarian carcinoma cell lines was studied. The Tat peptide mediated uptake resulted in cytoplasmic and nuclear localization and was found to be energy independent. Time and concentration studies verified the rapidity and dependence of the transport process on these parameters. Enhanced uptake of a polymer bound anticancer drug doxorubicin was also demonstrated. These results were corroborated independently by subcellular fractionation. (C) 2003 Elsevier B.V. All rights reserved.
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