期刊
TALANTA
卷 60, 期 6, 页码 1223-1228出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(03)00230-3
关键词
creatine; creatinine; amperometric biosensors; creatininase; creatinase; sorcosine oxidase
In order to determine creatine and creatinine amperometric biosensors were proposed. A bienzymatic biosensor based on creatinase (Cl) and sarcosine oxidase (SO) was used for the assay of creatine and a trienzymatic biosensor based on Cl. SO and creatininase (CA) for the assay of creatinine. The linear concentration ranges are of pmol l(-1) to nmol l(-1) magnitude order, with very low limits of detection. The biosensors proved high reliability for determination of creatine and creatinine as raw material, and in the pharmaceutical formulation. (C) 2003 Elsevier B.V. All rights reserved.
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