期刊
ARCHIVES OF PHARMACAL RESEARCH
卷 36, 期 11, 页码 1345-1353出版社
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-013-0162-y
关键词
Dipsaci Radix; Dipsacus asperoides; HPLC-UV; Quality control
资金
- National Center for Standardization of Herbal Medicine [09112KFDA817, 12172KFDA989]
- Korea Food Drug Administration, Republic of Korea
In this study, quantitative and pattern recognition analyses were developed using HPLC/UV for the quality evaluation of Dipsaci Radix. For quantitative analysis, five major bioactive compounds were assessed. The separation conditions employed for HPLC/UV were optimized using ODS C-18 column (250 x 4.6 mm, 5 mu m) with a gradient of acetonitrile and water as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 212 nm. These methods were fully validated with respect to linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of five major compounds in the extract of Dipsaci Radix. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of 17 Dipsaci Radix and four Phlomidis Radix samples. The results indicate that the established HPLC/UV method is suitable for quantitative analysis.
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