期刊
JOURNAL OF ORTHOPAEDIC RESEARCH
卷 21, 期 5, 页码 888-898出版社
WILEY
DOI: 10.1016/S0736-0266(03)00050-0
关键词
repetitive loads; articular cartilage; cell death; collagen damage; superficial tangential zone
类别
资金
- NIAMS NIH HHS [AR 45748, R01 AR045748] Funding Source: Medline
We investigated the effect of light (0.1 MPa), moderate (1 MPa) or heavy (5 MPa) cyclical stresses applied continuously or intermittently for 0 to 72 h on cell death and collagen damage in adult bovine cartilage explants. No increase in cell death was observed in the cartilage loaded with a continuous cyclic stress at 0.1 MPa for up to 72 h. Cell death occurred in the uppermost superficial tangential zone (STZ) of explants after loading for 1 h at 1 MPa, and reached a maximum depth of 61 23 mum by 6 h (at the rate of 9 6 mum/h). At 5 MPa, cell death occurred in the STZ after as little as 1 min (30 cycles) of loading, and reached a maximum depth of 70::E 2 mum by 60 min (47 8 mum/h). When an intermittent (with 2 s on, 2 s off) stress of 5 MPa was applied, cell death appeared in the STZ after 2 min (30 cycles) and increased to a depth of 63+/-2 pm at 60 min (45+/-11 mum/h). No significant differences were observed between the continuous and intermittent loading conditions. Both collagenase-cleaved and denatured collagen fibers were found in the STZ of explants loaded at 1 and 5 MPa. We concluded that load-induced cell death depends on load duration and magnitude, and that the chondrocytes in the STZ are more vulnerable to load-induced injury than those in the middle and deep zones. (C) 2003 Published by Elsevier Ltd. on behalf of Orthopaedic Research Society.
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