期刊
PHYTOPATHOLOGY
卷 93, 期 9, 页码 1145-1152出版社
AMER PHYTOPATHOLOGICAL SOC
DOI: 10.1094/PHYTO.2003.93.9.1145
关键词
biocontrol mechanisms; biological control; gray mold; hydrolytic enzyme; mycoparasitism; postharvest apples
The PaEXG2 gene. encoding an exo-beta-1,3-glucanase, was isolated from the biocontrol agent Pichia anomala strain K. PaEXG2 has the capacity for coding an acidic protein of 427 amino acids with a predicted molecular weight of 45.7 kDa. a calculated pI of 4.7, and one potential N-glycosylation site. PaEXG2 was disrupted by the insertion of the URA3 marker gene. encoding orotidine monophosphate decarboxylase in strain KUI, a uracil auxotroph derived from strain K. Strain KUI showed inferior biocontrol activity and colonization of wounds on apples, compared to the prototrophic strain. Antagonism and colonization were recovered after the restoration of prototrophy by transformation with the URA3 gene. Integrative transformation was shown to be mostly ectopic in strain K descendants (only 4% of integration by homologous recombination). PaEXG2 disruption abolished all detectable extracellular exo-p1,3-glucanase activity in vitro and in situ but did not affect biocontrol of Botrytis cinerea on wounded apples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据