期刊
JOURNAL OF PEPTIDE SCIENCE
卷 9, 期 9, 页码 574-593出版社
WILEY
DOI: 10.1002/psc.475
关键词
chemical protein synthesis; enzymes; chemical ligation; native chemical ligation; peptide synthesis; peptide thioester; solid phase peptide synthesis; solid phase chemical ligation; backbone engineering; HIV-1 protease
The total synthesis, at will, of a wide variety of protein and enzyme molecules is made feasible by modern chemical ligation methods. As Emil Fischer intuitively understood, synthetic access to the enzyme molecule enables the power of chemical science to be applied to elucidating the molecular basis of catalytic function in unprecedented detail. Copyright (C) 2003 European Peptide Society and John Wiley Sons, Ltd.
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