期刊
JOURNAL OF CELL BIOLOGY
卷 162, 期 5, 页码 781-787出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.200303144
关键词
chemotaxis; receptor; epidermal growth factor; signal transduction; EGF bead
类别
资金
- NCI NIH HHS [R01 CA077522, T32 CA009475, 5T32-CA09475, CA77522] Funding Source: Medline
- NIDDK NIH HHS [T32 DK007513, 5T32-DK07513] Funding Source: Medline
- NIGMS NIH HHS [R01 GM055692, GM55692] Funding Source: Medline
Differing spatial scales of signaling cascades are critical for cell orientation during chemotactic responses. We used biotin EGF bound to streptavidin-coupled magnetic beads to locally Stimulate cells overexpressing the EGF receptor. We have found that EGF-induced actin polymerization remains localized even under conditions of receptor overexpression. Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation. The localized actin polymerization is independent of P13-kinase and rho protein activity and requires Arp2/3 complex and cofilin function. Thus, we find differing spatial scales of signaling from the EGF receptor, supporting models of chemotaxis that integrate short- and long-range signaling.
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