期刊
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
卷 54, 期 1, 页码 14-24出版社
WILEY-LISS
DOI: 10.1002/arch.10095
关键词
LpR(Gm); ligand blot analysis; HDLp (high density lipophorin); Galleria mellonella
To identify and characterize the HDLp (high-density lipophorin) receptor from Galleria mellonella (LpR(Gm)), we used techniques of ligand blotting. This method was, to our knowledge, first used to characterize the lipophorin receptor (LpR) in insects. LpR(Gm) had an approximate molecular weight of 97 kDa under non-reducing conditions and bound the HDLp specifically. The time-course of lipophorin binding to their receptor protein was rapid. The binding of lipophorins to their receptors was saturable with a K-d of 34.33 +/- 4.67 mug/ml. Although Ca2+ was essentially required in the binding of HDLp to their receptors, interestingly increasing concentration of Ca2+ has shown to have a slight inhibitory effect. EDTA was used here as Ca2+ chelating reagent, because Mg2+ in the binding buffer did not affect the binding of HDLp to their receptors, and inhibited the binding of HDLp and LpR(Gm) absolutely. Suramin (polysulfated polycyclic hydrocarbon), known to inhibit the binding of lipoproteins to their receptors, effectively abolished the binding of HDLp to their receptors. LpR(Gm) showed the stage specific binding activity especially in day 1-3 lost instar larval, prepupol, and day 1-3 adult stages. (C) 2003 Wiley-Liss, Inc.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据