期刊
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
卷 446, 期 6, 页码 684-694出版社
SPRINGER-VERLAG
DOI: 10.1007/s00424-003-1111-2
关键词
M-current; KCNQ channels; dopamine D-2 receptors; electrophysiology; Xenopus laevis oocytes; SH-SY5Y cells; quinpirole; Bordetella pertussis toxin
类别
Activation of KCNQ potassium channels by stimulation of co-expressed dopamine D-2 receptors was studied electrophysiologically in Xenopus laevis oocytes and in mammalian cells. To address the specificity of the interaction between D-2-like receptors and KCNQ channels, combinations of KCNQ1-5 channels and D-2-like receptors (D-2L, D-3, and D-4) were investigated in Xenopus oocytes. Activation of either receptor with the selective D-2-like receptor agonist quinpirole (100 nM) stimulated all the KCNQ currents, independently of the subunit combination, indicating a common pathway of receptor-channel interaction. The KCNQ4 current was investigated in further detail and was increased by 19.9+/-1.6% (n=20) by D-2L receptor stimulation. The effect could be mimicked by injection of GTPgammaS and prevented by injection of Bordetella pertussis toxin, indicating that channel stimulation was mediated via a G protein of the G(alphai/o) subtype. Cells of the human neuroblastoma line SH-SY5Y were co-transfected transiently with KCNQ4 and D-2L receptors. Stimulation of D-2L receptors increased the KCNQ4 current (n=6) as determined in whole-cell patch-clamp recordings. The specificity of the dopaminergic activation of the KCNQ channels was confirmed by co-expression of other neuronal K+ channels (BK, K(V)1.1, and K(V)4.3) with the D-2L receptor in Xenopus oocytes. None of these K+ channels responded to stimulation of the D-2L receptor. In the mammalian brain, dopamine D-2 receptors and KCNQ channels co-localise postsynaptically in several brain regions, so modulation of neuronal excitability by dopamine release could in part be mediated via an effect on KCNQ channels.
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