Functional CD5+ B cells develop predominantly in the spleen of NOD/SCID/γcnull (NOG) mice transplanted either with human umbilical cord blood, bone marrow, or mobilized peripheral blood CD34+ cells

Objective. Human CD5(+) B cells are the major B cell subset in fetal spleen and umbilical cord blood (CB), and their number gradually diminishes in both spleen and peripheral blood from infancy through childhood while conventional B cells increase. In this study, we investigated whether CD5(+) cells differentiate from adult hematopoietic stem cells (HSCs) as well as fetal ones in immunodeficient mice. Methods. In our system, NOD/SCID/gammac(null) (NOG) mice were transplanted with CD34(+) cells from CB (hCB model), adult bone marrow (hBM model), and mobilized peripheral blood (hMPB model). Results. In these model mice, a high proportion of CD19(+)IgM(+)CD5(+) mature B cells appeared in the spleen, regardless of the CD34(+) cell origin, 4 to 8 weeks after transplantation, while the majority were CD19(+)IgM(-)CD5(-) immature B cells in BM. The CD19(+)CD5(-) BM cells showed to express CD5 after the coculture with NOG spleen cells. In the sera of immunized hCB model mice with DNP-KLH, antigen-specific IgM but not IgG was enhanced. Conclusion. Our results show that adult CD34(+) cells develop into functional CD5(+) B cells in NOG spleen as much as fetal CD34(+) cells do. (C) 2003 International Society for Experimental Hematology. Published by Elsevier Inc.