期刊
PLANT JOURNAL
卷 35, 期 5, 页码 604-612出版社
BLACKWELL PUBLISHING LTD
DOI: 10.1046/j.1365-313X.2003.01832.x
关键词
recombination; gene conversion; single-strand annealing; synthesis-dependent strand-annealing deletion
Different DNA repair pathways that use homologous sequences in close proximity to genomic double-strand breaks (DSBs) result in either an internal deletion or a gene conversion. We determined the efficiency of these pathways in somatic plant cells of transgenic Arabidopsis lines by monitoring the restoration of the P-glucuronidase (GUS) marker gene. The transgenes contain a recognition site for the restriction endonuclease I-Scel either between direct GUS repeats to detect deletion formation (DGU.US), or within the GUS gene to detect gene conversion using a nearby donor sequence in direct or inverted orientation (DU.GUS and IU.GUS). Without expression of I-Scel, the frequency of homologous recombination (HR) was low and similar for all three constructs. By crossing the different lines with an I-Scel expressing line, DSB repair was induced, and resulted in one to two orders of magnitude higher recombination frequency. The frequencies obtained with the DGU.US construct were about five times higher than those obtained with DU.GUS and lU.GUS, irrespective of the orientation of the donor sequence. Our results indicate that recombination associated with deletions is the most efficient pathway of homologous DSB repair in plants. However, DSB-induced gene conversion seems to be frequent enough to play a significant role in the evolution of tandemly arranged gene families like resistance genes.
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