期刊
GROWTH FACTORS
卷 21, 期 3-4, 页码 151-160出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/08977190310001636595
关键词
PDGF; vitamin D receptor; transcription; DNA elements
资金
- NCI NIH HHS [CA83237-02] Funding Source: Medline
- NHLBI NIH HHS [HL62877-01A1] Funding Source: Medline
- NIA NIH HHS [AG10836-06S1] Funding Source: Medline
Expression of the platelet-derived growth factor-A subunit (PDGF-A) is regulated to a significant degree by DNA elements located in the 5'-distal region of the gene. A potent basal enhancer (ACE66) located approximately 7 kb upstream of the transcription start site contains a number of half-sites for nuclear receptor binding, two of which are arranged in the form of direct repeat-3 motif that corresponds to a consensus vitamin D response element (VDRE). Electrophoretic mobility shift assays confirmed that the ACE66 sequence was recognized as a high affinity target for binding of heterodimers of recombinant vitamin D receptor (VDR) and its partner, retinoid-X receptor-alpha (RXRalpha). VDRE activity was localized by transient transfection analysis to a direct repeat-3 motif within the 5'-portion of the ACE66 element. Moreover, 1,25-(OH) 2 D 3 was validated as a regulator of the endogenous PDGF-A gene by the vitamin D-stimulated upregulation of PDGF-A mRNA levels in a VDR-expressing clone of JEG-3 cells. Thus, PDGF-A represents a novel mitogenic target of 1,25-(OH) 2 D 3 whose expression is induced via binding of hormone-activated VDR to a response element located far upstream of the transcription start site.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据