期刊
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
卷 79, 期 9, 页码 701-708出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09553000310001606803
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Purpose: International thresholds for exposure to non-ionizing radiation leading to non-thermal effects were conservatively set by the International Commission on Non-Ionizing Radiation Protection (ICNIRP). The aim of this study was to examine whether biological effects such as different modes of cell death and gene expression modifications related to tumorgenesis are detectable above the threshold defined. Materials and methods: Human leukaemia cells (HL-60) grown in vitro were exposed to electromagnetic fields (EMF; t(1/2r) about 1 ns; field strength about 25 times higher than the ICNIRP reference levels for occupational exposure) leading to non-thermal effects using a high-voltage-improved GTEM cell 5302 (EMCO) connected to a pulse generator NP20 (C=1 nF, U-Load=20 kV). HL-60 cells were harvested at 0, 24, 48 and 72 h after radiation exposure. Micronuclei, apoptosis and abnormal cells (e.g. necrosis) were determined using morphological criteria. In parallel, the expression of 1176 genes was measured using Atlas(TM) Human 1.2. Array. Based on high data reproducibility calculated from two independent experiments (>99%), array analysis was performed. Results: No significant change in apoptosis, micronucleation, abnormal cells and differential gene expression was found. Conclusions: Exposure of HL-60 cells to EMFs 25 times higher than the ICNIRP reference levels for occupational exposure failed to induce any changes in apoptosis, micronucleation, abnormal morphologies and gene expression. Further experiments using EMFs above the conservatively defined reference level set by the ICNIRP may be desirable.
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