期刊
PHYTOCHEMISTRY
卷 64, 期 2, 页码 401-409出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(03)00269-3
关键词
Catharanthus roseus; Apocynaceae; tabersonine; lochnericine; horhammericine; hairy roots; methyl jasmonate; epoxidation; cytochrome P-450
Methyl jasmonate, a chemical inducer of secondary metabolism, was shown to promote tabersonine 2 biosynthesis in hairy root cultures of Catharanthus roseus. Tabersonine 6,7-epoxidase activity was detected in total protein extract of jasmonate-induced hairy root cultures using labeled C-14-tabersonine 2. This enzyme converted tabersonine 2 to lochnericine 3 by selective epoxidation at positions 6 and 7 via a reaction dependent on NADPH and molecular oxygen. Carbon monoxide, clotrimazole, miconazole, and cytochrome C were shown to be strong inhibitors of the enzyme. The activity was found in microsomes, indicating that tabersonine 6,7-epoxidase was a cytochrome P-450-dependent monooxygenase. (C) 2003 Elsevier Ltd. All rights reserved.
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