期刊
JOURNAL OF NEUROCHEMISTRY
卷 86, 期 6, 页码 1498-1505出版社
WILEY
DOI: 10.1046/j.1471-4159.2003.01953.x
关键词
astrocyte; L-glutamate transporter; estrogen; xenoestrogen; membrane ER alpha; E2-BSA
We investigated the effects of estrogen-related compounds including xenoestrogens [17beta-estradiol (E2), 17alpha-ethynylestradiol (EE), diethylstilbestrol (DES), p-nonylphenol (PNP), bisphenol A (BPA) and 17alpha-estradiol (17alpha)] on L-glu uptake by cultured astrocytes via glutamate-aspartate transporter (GLAST). After 24 h treatment, E2 inhibited the L-glu uptake at 1 mum and higher concentrations. EE and DES also inhibited the L-glu uptake at 1 nm and higher concentrations. The other four compounds had no effect. The effects of E2, EE and DES were completely blocked by 10 nm of IC1182 780 (ICI). beta-Estradiol 17-hemisuccinate : bovine serum albumin (E2-BSA), a membrane-impermeable conjugate of E2, also elicited the inhibition of L-glu uptake at 1 mum and higher concentrations, and the effect was blocked by ICI. 16alpha-lodo-17beta-estradiol (16alphaIE2), an estrogen receptor alpha (ERalpha) selective ligand, revealed an inhibitory effect at 10 nm, while genistein, an ERbeta selective ligand, failed to reveal such an effect at this concentration. Western blot analysis showed that the predominant ER of cultured astrocytes was ERalpha. The colocalization of ERalpha with GLAST on plasma membranes was immunohistochemically detected in these cells. From these results, we concluded that estrogens down-regulate L-glu uptake activity of astrocytes via membrane ERalpha.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据