期刊
ARCHIVES OF ORAL BIOLOGY
卷 57, 期 10, 页码 1377-1384出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.archoralbio.2012.07.009
关键词
Mechanical stress; PGE(2); RANKL; Osteoclastogenesis; PDL
资金
- Ministry of Education, Science, Culture, Sports, Science and Technology [S1001024]
- Dental Research Centre
- Sato Fund
- Nihon University School of Dentistry
- The Promotion and Mutual Aid Corporation for Private Schools of Japan
- [22592298]
Objective: Periodontal ligament cells (PDLs) produce prostaglandin E-2 (PGE(2)) in response to orthodontic force. PGE(2) is a potent osteoclast-inducing factor that induces the receptor activator of nuclear factor-KB ligand (RANKL). Some studies reported that PDLs express RANKL in response to mechanical stress, whereas another study reported that they do not. Based on an immunohistochemical study, RANKL expression is localized around the alveolar bone surface 3 days after tooth movement. However, ankylosed teeth cannot be moved by therapeutic mechanical stress, suggesting that PDLs play a major role in alveolar bone resorption. In this study, we compared the functional difference in osteoclastogenesis between human PDLs (HPDLs) and normal human osteoblasts (HOBs) as a direct effect of PGE(2) exposure. Design: We examined the expression of RANKL, osteoprotegerin, and macrophage colonystimulating factor after 48-h culture with or without PGE(2) (10(-11) to 10(-5) M) in HPDLs and HOBs. Then to confirm whether RANKL produced by PGE(2) treatment induces osteoclastogenesis or not, RAW264.7 cells were co-cultured on HPDLs or HOBs pretreated with 10(-6) M of PGE(2). Result: PGE(2) exposure increased significantly RANKL expression in HOBs compared with HPDLs. PGE(2) exposure significantly decreased osteoprotegerin expression in HPDLs compared with HOBs. The number of tartrate-resistant acid phosphatase staining osteoclastlike cells from RAW264.7 cells increased significantly by PGE(2) pretreatment in HOBs and was reduced by small interfering RNA knockdown of RANKL. Conclusion: These results suggest that osteoblasts strongly influence the stimulation of osteoclastogenesis via RANKL, induced by PGE(2) in periodontal tissues, compared with PDLs. (c) 2012 Elsevier Ltd. All rights reserved.
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