期刊
GENESIS
卷 37, 期 1, 页码 18-24出版社
WILEY
DOI: 10.1002/gene.10218
关键词
embryonic stem cells; Oct4; RNA interference; trophoblast; differentiation
资金
- NIDCR NIH HHS [DE07057] Funding Source: Medline
- NINDS NIH HHS [NS39438] Funding Source: Medline
- PHS HHS [F005952, F005192] Funding Source: Medline
We examined whether suppression of Oct4 via RNA interference (RNAi) would affect embryonic stem (ES) cell lineage choice: Cells were transfected with plasmids containing an independently expressed reporter gene and an RNA polymerase type III promoter to constitutively express small stem-loop RNA transcripts corresponding to Oct4 mRNA. Cells transfected with Oct4 RNAi constructs demonstrated reduced levels of Oct4 mRNA and exhibited characteristics of trophectodermal differentiation. These findings support the critical role of Oct4 in regulating stem cell identity and suggest that future experiments using RNAi in ES cells can elucidate the roles of other genes affecting lineage specification during differentiation. (C) 2003 Wiley-Liss, Inc.
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