期刊
EMBO JOURNAL
卷 22, 期 17, 页码 4555-4565出版社
WILEY
DOI: 10.1093/emboj/cdg433
关键词
DNA replication; retrotransposon; reverse transcriptase; ribozyme
资金
- NIGMS NIH HHS [R01 GM037949, GM37949] Funding Source: Medline
The Lactococcus lactis Ll.LtrB group II intron uses a major retrohoming mechanism in which the excised intron RNA reverse splices into one strand of a DNA target site, while the intron-encoded protein uses a C-terminal DNA endonuclease domain to cleave the opposite strand and then uses the cleaved 3' end as a primer for reverse transcription of the inserted intron RNA. Here, experiments with mutant introns and target sites indicate that Ll.LtrB can retrohome without second-strand cleavage by using a nascent strand at a DNA replication fork as the primer for reverse transcription. This mechanism connecting intron mobility to target DNA replication may be used by group II intron species that encode proteins lacking the C-terminal DNA endonuclease domain and for group II intron retrotransposition to ectopic sites.
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