期刊
JOURNAL OF BIOTECHNOLOGY
卷 104, 期 1-3, 页码 129-153出版社
ELSEVIER
DOI: 10.1016/S0168-1656(03)00144-5
关键词
mebacterium glutamicum; cytochrome aa(3) oxidase; cytochrome bc(1) complex; dehydrogenase; electron transport chain; menaquinone; oxidative phosphorylation; respiration; respiratory chain; supercomplex
Corynebacterium glutamicum is an aerobic bacterium that requires oxygen as exogenous electron acceptor for respiration. Recent molecular and biochemical analyses together with information obtained from the genome sequence showed that C glutamicum possesses a branched electron transport chain to oxygen with some remarkable features. Reducing equivalents obtained by the oxidation of various substrates are transferred to menaquinone via at least eight different dehydrogenases, i.e. NADH dehydrogenase, succinate dehydrogenase, malate:quinone oxidoreductase, pyruvate:quinone oxidoreductase, D-lactate dehydrogenase, L-lactate dehydrogenase, glycerol-3-phosphate dehydrogenase and L-proline dehydrogenase. All these enzymes contain a flavin cofactor and, except succinate dehydrogenase, are single subunit peripheral membrane proteins located inside the cell. From menaquinol, the electrons are passed either via the cytochrome be, complex to the aa(3)-type cytochrome e oxidase with low oxygen affinity, or to the cytochrome bd-type menaquinol oxidase with high oxygen affinity. The former branch is exceptional, in that it does not involve a separate cytochrome c for electron transfer from cytochrome c(1) to the Cu-A center in subunit II of cytochrome aa(3). Rather, cytochrome cl contains two covalently bound heme groups, one of which presumably takes over the function of a separate cytochrome c. The be, complex and cytochrome aa3 oxidase form a supercomplex in C. glutamicum. The phenotype of defined mutants revealed that the bc(1)-aa(3) branch, but not the bd branch, is of major importance for aerobic growth in minimal medium. Changes of the efficiency of oxidative phosphorylation caused by qualitative changes of the respiratory chain or by a defective F1F0-ATP synthase were found to have strong effects on metabolism and amino acid production. Therefore, the system of oxidative phosphorylation represents an attractive target for improving amino acid productivity of C glutamicum by metabolic engineering. (C) 2003 Elsevier B.V. All rights reserved.
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