期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 332, 期 1, 页码 171-182出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/S0022-2836(03)00899-4
关键词
voltage-gated calcium channel subunits; immuno labeling; single-particle image processing; tetrad; excitation-contraction coupling complex
资金
- NIGMS NIH HHS [1 P01 GM-62580] Funding Source: Medline
The three-dimensional structure of the skeletal muscle voltage-gated L-type calcium charnel (Ca(v)1.1; dihydropyridine receptor, DHPR) was determined using electron cryo-microscopy and single-particle averaging. The structure shows a single channel complex with an approximate total molecular mass of 550 kDa, corresponding to the five known subunits of the DHPR, and bound detergent and lipid. Features visible in our structure together with antibody labeling of the beta and alpha(2) subunits allowed us to assign locations for four of the five subunits within the structure. The most striking feature of the structure is the extra-cellular alpha(2) subunit that protrudes from the membrane domain in close proximity to the alpha(2) subunit. The cytosolic beta subunit is located close to the membrane and adjacent to subunits alpha(1), gamma and delta. Our structure correlates well with the functional and biochemical data available for this channel and suggests a three-dimensional model for the excitation-contraction coupling complex consisting of DHPR tetrads and the calcium release channel. (C) 2003 Elsevier Ltd. All rights reserved.
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