期刊
LIFE SCIENCES
卷 73, 期 16, 页码 2019-2028出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0024-3205(03)00565-4
关键词
cGMP; PKG; SERCA; endothelial cells
Calcium is a crucial regulator of many physiological processes such as cell growth, division, differentiation, cell death and apoptosis. In this study, we examined the effect of cGMP on agonist-induced [Ca2+](i) transient in isolated rat aortic endothelial cells. 100 muM ATP was applied to the cells bathed in a Ca2+-free physiological solution to induce a [Ca2+](i) transient that was caused by Ca2+ release from intracellular stores. cGMP, which was applied after [Ca2+](i) reached its peak level, accelerated the falling phase of [Ca2+](i) transient. Pre-treatment of the cells with CPA abolished the accelerating effect of cGMP on the falling phase of [Ca2+](i) transient. The effect of cGMP was reversed by KT5823, a highly specific inhibitor of protein kinase G. Taken together, these data suggest that cGMP may reduce [Ca2+](i) level by promoting Ca2+ uptake through sarcoplasmic/endoplasmic reticulum ATPase and that the effect of cGMP may be mediated by protein kinase G. (C) 2003 Elsevier Inc. All rights reserved.
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