期刊
FEBS LETTERS
卷 551, 期 1-3, 页码 153-158出版社
WILEY
DOI: 10.1016/S0014-5793(03)00917-7
关键词
caspase; endothelial cell; GTPase p21ras; S-nitrosylation; shear stress; thioredoxin
Laminar flow (shear stress) is an important stimulus for nitric oxide (NO) synthesis in endothelial cells. NO can react with free SH-groups of different proteins leading to S-nitrosylation. Since S-nitrosylation of proteins is an important regulator of protein functions, we investigated the effect of endogenously synthesized NO. Exposure to shear stress significantly increased the overall S-nitrosylation of proteins in endothelial cells. Interestingly, shear stress increased S-nitrosylation of specific target proteins, i.e. the catalytic p17 subunit of caspase-3, the GTPase p21ras and the oxidoreductase thioredoxin. S-nitrosylation resulted in an inhibition of caspase-3 and in an augmented activity of p21ras and thioredoxin. These data suggest that long term exposure to shear stress exerts its different atheroprotective effects at least in part via increased S-nitrosylation of specific signaling proteins. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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