期刊
FEBS LETTERS
卷 551, 期 1-3, 页码 78-86出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00900-1
关键词
MIF; cytokine; nonclassical secretion; leaderless secretory protein; ABC transporter
资金
- NIAID NIH HHS [2R01-AI42310] Funding Source: Medline
The cytokine macrophage migration inhibitory factor (MIF) is inducibly secreted by immune cells and certain other cell types to critically participate in the regulation of the host immune response. However, MIF does not contain a N-terminal signal sequence and the mechanism of MIF secretion is unknown. Here we show in a model of endotoxin-stimulated THP-1 monocytes that MIF does not enter the endoplasmatic reticulum and that MIF secretion is not inhibited by monensin or brefeldin A, demonstrating that MIF secretion occurs via a non-classical export route. Glyburide and probenicide but not other typical inhibitors of non-classical protein export strongly block MIF secretion, indicating that the export pathway of MIF involves an ABCA1 transporter. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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