期刊
AQUACULTURE RESEARCH
卷 34, 期 11, 页码 951-957出版社
WILEY
DOI: 10.1046/j.1365-2109.2003.00949.x
关键词
dragonfish; bonytongue; AFLP; RAPD; molecular sexing; strain identification
类别
A sex-associated amplified fragment length polymorphism and a strain-specific random amplified polymorphic DNA marker were identified from Asian arowana (dragonfish; Scleropages formosus Muller & Schlegel) by screening pooled genomic DNA samples from three different strains as well as males and females respectively. Both markers were cloned, sequenced and successfully converted into sequence-tagged-site (STS) markers. The strain-specific STS marker could be applied to differentiate the Indonesian golden strain of Asian arowana from the green and blood-red strains before the stage when colours become identifiable. Individuals from the green strain could be sexed with an efficiency of 82.7% using the sex-associated STS marker. Thus, populations with preferred sex ratios can be formed without the need of rearing a large number of fish.
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