期刊
FEBS LETTERS
卷 552, 期 2-3, 页码 240-246出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0014-5793(03)00931-1
关键词
lipid phosphate phosphatase; phosphatidic acid phosphatase (type 2); MDCK cell; apical sorting signal; basolateral surface
Lipid phosphate phosphatases (LPPs) are integral membrane proteins with six transmembrane domains that act as ecto-enzymes dephosphorylating a variety of extracellular lipid phosphates. Using polarized MDCK cells stably expressing human LPP1 and LPP3, we found that LPP1 was located exclusively at the apical surface whereas LPP3 was distributed mostly in the basolateral subdomain. We identified a novel apical sorting signal at the N-terminus of LPP1 composed of F(2)DKTRL(7). In the case of LPP3, a dityrosine motif present in the second cytoplasmic portion was identified as basolateral targeting signal. Our work shows that LPP1 and LPP3 are equipped with distinct sorting signals that cause them to differentially localize to the apical vs. the basolateral subdomain, respectively. (C) 2003 Published by Elsevier B.V. All rights reserved.
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