Binding of Porphyromonas gingivalis fimbriae to Treponema denticola dentilisin

Treponema denticola has been reported to coaggregate with Porphyromonas gingivalis and localize closely together in matured subgingival plaque. In this study of the interaction of T denticola with P. gingivalis, the P. gingivalis fimbria-binding protein of T denticola was identified by two-dimensional electrophoresis followed by a ligand overlay assay with P. gingivalis fimbriae, and was determined to be dentilisin, a chymotrypsin-like proteinase of T denticola. The binding was further demonstrated with a ligand overlay assay using an isolated GST fusion dentilisin construct. Our results suggest that P. gingivalis fimbriae and T denticola dentilisin are implicated in the coaggregation of these bacteria. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.