期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 207, 期 1-2, 页码 39-45出版社
ELSEVIER SCI IRELAND LTD
DOI: 10.1016/S0303-7207(03)00257-0
关键词
LRH-1; SF-1; aromatase; ovary; granulosa; cell; luteal cell
资金
- NIDDK NIH HHS [5-R01-DK31206] Funding Source: Medline
Steroid biosynthesis in ovary is enhanced by the orphan nuclear receptor, steroidogenic factor-1 (SF-1); however, we reported that liver receptor homolog-1 (LRH-1), a closely related receptor to SF-1, is also expressed in mouse ovary. To further investigate the role of LRH-1 in mouse ovary, we used in situ hybridization to identify the cell types that express LRH-1 versus SF-1, and carried out functional studies to determine the role of LRH-1 in the regulation of the human (h) ovary-specific CYP19 promoter. LRH-1 expression was found to be abundant and highly restricted to cells involved in estrogen biosynthesis-granulosa cells during the estrous cycle, and in corpora lutea (CL) of pregnancy. In contrast, SF-1 was expressed most highly in C-19-steroid-producing theca cells and interstitium, and at low levels in granulosa and luteal cells. Transfection studies using gramilosa cells demonstrated that LRH-1 is a potent regulator of both basal and forskolin-induced transcription of the ovary-specific hCYP19 promoter. This activity was dependent upon two nuclear receptor half-sites within the proximal hCYP19 promoter. Based on these findings, we propose that LRH-1 plays an important role as a competence factor in regulating aromatase, and thus estrogen biosynthesis, in ovary. (C) 2003 Elsevier Ireland Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据