期刊
ELECTROPHORESIS
卷 24, 期 19-20, 页码 3305-3313出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200305590
关键词
inductively coupled plasma-mass spectrometry laser ablation; metal-protein complexes; selenium
The capabilities of laser ablation-inductively coupled plasma-mass spectrometry for the detection of trace elements in a gel after gel electrophoresis were systematically studied. Figures of merit, such as limit of detection, linearity, and repeatability, were evaluated for various elements (Li, V, Cr, Mn, Ni, Cu, Zn, As, Se, Mo, Pd, Ag, Cd, Pt, Tl, Pb). Two ablation strategies were followed: single hole drilling, relevant for ablation of spots after two-dimensional (2-D) separations, and ablation with translation, i.e., on a line, relevant for one-dimensional (1-D) separations. This technique was applied to the detection of selenoproteins in red blood cells extracts after a 1-D separation (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) and the detection of selenium-containing proteins in yeast after 2-D electrophoresis (2-DE). The detection procedure was further improved by using the dynamic reaction cell technology, which allowed the removal of the Ar-2(+) interference and hence the use of the most abundant Se isotope, Se-80. Reaction gases were compared (methane, carbon monoxide, ammonia, oxygen and the combination of argon (collision gas) and hydrogen (reaction gas)). In each instance, the reaction cell parameters were optimized in order to obtain the lowest detection limit for Se (as Se-80(+), Se-82(+) or Se-77(+); and as Se-80 O-16(+), (SeO+)-Se-82-O-16 or (SeO+)-Se-77-O-16 with O-2 as the reaction gas). Carbon monoxide was found to offer the best performance. The detection limit with the use of DRC and He as transport gas was 0.07 mug Se g(-1) gel with single hole drilling and 0.15 mug Se g(-1) gel for ablation with translation.
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