期刊
PLANT CELL TISSUE AND ORGAN CULTURE
卷 75, 期 1, 页码 87-94出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1024684605967
关键词
Mentha arvensis L.; menthol tolerance; mint oil; new variety; RAPD; somaclones; yield
In vitro raised shoots of Mentha arvensis L. were screened for menthol tolerance level by growing them in media containing 0-100 mug ml(-1) menthol. A total of 2850 regenerated shoots were step wise screened for menthol tolerance at the concentrations of 50 mug ml(-1) followed by 60 and 70 mug ml(-1). In this screening, only 30 individual regenerated shoots were able to survive. The clones from the primary screen were inoculated into rooting medium and, after rooting, transferred to pots in the greenhouse. Ultimately, these 30 menthol tolerant clones were multiplied and grown in the field in replicated plots of 2.5 X 2.5 m sizes. Twigs of 30 clones from the replicated trials were rechecked for tolerant phenotypes at a concentration of 70 mug ml(-1) menthol wherein, these survived even after 7 days ( secondary screening). These clones were checked for oil and menthol content and were found to be better than the control plants. Out of these 30 plants, five tolerated 80 mug ml(-1) menthol ( tertiary level screening) and were found to contain the highest amount of menthol per g leaf biomass. Molecular analysis through RAPD showed distinct variation in the profiles of these five plants, in comparison to the control. Using this method the relationship between the primer OPT 04, menthol tolerance and high menthol content character of the genotype was established. Further, a cultivar 'Saksham' was released from the selections by CIMAP for superior performance.
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