期刊
BIOLOGY OF REPRODUCTION
卷 69, 期 4, 页码 1213-1219出版社
OXFORD UNIV PRESS INC
DOI: 10.1095/biolreprod.102.015164
关键词
estradiol; follicular development; granulosa cells; in vitro fertilization; insulin
资金
- NCRR NIH HHS [RR13635, RR14093, RR00167] Funding Source: Medline
Five early-treated and four late-treated prenatally androgenized and five normal female rhesus monkeys were studied to determine whether prenatal testosterone propionate exposure beginning Gestational Days 40-44 (early-treated) or 100-115 (late-treated) affects follicular steroidogenesis during recombinant human FSH (rhFSH) treatment. All monkeys underwent rhFSH injections, without human chorionic gonadotropin administration, followed by oocyte retrieval. Serum FSH, LH, estradiol (E-2), progesterone (P), 17alpha-hydroxyprogesterone (17 OHP), androstenedione (A(4)), testosterone, and dihydrotestosterone were measured basally during rhFSH therapy and at oocyte retrieval. Follicle fluid (FF) sex steroids, oocyte fertilization, and embryo development were analyzed. Circulating FSH, E-2, 17 OHP, A(4), and dihydrotestosterone levels increased similarly in all females. Serum LH levels decreased from basal levels in normal and late-treated prenatally androgenized females but were unchanged in early-treated prenatally androgenized females. Serum P levels at oocyte retrieval were comparable with those before FSH treatment in all females. All prenatally androgenized females showed reduced FF levels of A(4) and E-2 but not P or dihydrotestosterone. Intrafollicular T concentrations also were significantly lower in late-treated compared with early-treated prenatally androgenized females or normal females. In early-treated prenatally androgenized females, but not the other female groups, intrafollicular A(4) and E-2 levels were reduced in follicles containing oocytes that failed fertilization or produced zygotes with cleavage arrest before or at the five- to eight-cell embryo stage. Therefore, in monkeys receiving rhFSH therapy alone without human chorionic gonadotropin administration, early prenatal androgenization reduced FF concentrations of E-2 and A(4) in association with abnormal oocyte development, without having an effect on P, testosterone, or dihydrotestosterone concentrations.
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