Immunomodulatory activity of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465

Bacterial polysaccharides are promising stimulants of protective functions in humans and animals. We investigated the ability of exopolysaccharide from the rhizobacterium Paenibacillus polymyxa CCM 1465 to induce nonspecific resistance factors in the macroorganism. We examined in vitro the effect of the exopolysaccharide, produced with different carbon sources, on the phagocytic activity of murine macrophages, on the generation of reactive oxygen species and of enzymes (acid phosphatase and myeloperoxidase), on the proliferation of murine splenocytes, and on the synthesis of proinflammatory cytokines [interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha)] by human mononuclear cells. The exopolysaccharide promoted the phagocytosis of bacterial cells, activated metabolic processes in human and animal leukocytes, and moderately affected the production of TNF-alpha and IL-1 beta. The exopolysaccharides produced on media with glucose and sucrose differed in their effect on the immune cells, possibly owing to their different compositions, structures, and properties. The results validly indicate that the exopolysaccharide of P. polymyxa CCM 1465 promotes nonspecific immunity. Therefore, it can find application as a biologically active immunomodulatory substance.