期刊
ARCHIVES OF MICROBIOLOGY
卷 195, 期 10-11, 页码 717-726出版社
SPRINGER
DOI: 10.1007/s00203-013-0922-6
关键词
PhyR; General stress response; Phyllosphere; Methane monooxygenase; Methanol
类别
资金
- Japan Society for the Promotion of Science [22380052, 22310046]
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [22310046, 25281063] Funding Source: KAKEN
Methanotrophs are widespread and have been isolated from various environments including the phyllosphere. In this study, we characterized the plant colonization by Methylosinus sp. B4S, an alpha-proteobacterial methanotroph isolated from plant leaf. The gfp-tagged Methylosinus sp. B4S cells were observed to colonize Arabidopsis leaf surfaces by forming aggregates. We cloned and sequenced the general stress response genes, phyR, nepR and ecfG, from Methylosinus sp. B4S. In vitro analysis showed that the phyR expression level was increased after heat shock challenge, and phyR was shown to be involved in resistance to heat shock and UV light. In the phyllospheric condition, the gene expression level of phyR as well as mmoX and mxaF was found to be relatively high, compared with methane-grown liquid cultures. The phyR-deletion strain as well as the wild-type strain inoculated on Arabidopsis leaves proliferated at the initial phase and then gradually decreased during plant colonization. These results have shed light firstly on the importance of general stress resistance and C1 metabolism in methanotroph living in the phyllosphere.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据