期刊
ARCHIVES OF MICROBIOLOGY
卷 195, 期 3, 页码 197-202出版社
SPRINGER
DOI: 10.1007/s00203-013-0867-9
关键词
Bacillus subtilis; Spore surface display; Biocatalyst; beta-Galactosidase; Spore coat proteins
类别
资金
- National Institute of Genetics Engineering and Biotechnology, Iran
- National Centre for Research and Development, Poland [NR12 0143 10]
The production of highly efficient, recyclable and cost-effective enzymes is one of the most important goals in industrial biotechnology. Bacterial spores are highly resistant to harsh environmental conditions, easy to produce and are suitable for manipulation of genetic materials. These features make them a very efficient tool for biotechnology. Here, we show the use bacterial spores for presentation of functional enzyme. Spore coat display was used to produce a biocatalyst, which expresses beta-galactiosidase (LacA). This enzyme is commonly used to produce lactose-free milk for lactose intolerant individuals. The lacA gene from Bacillus subtilis strain 168 was expressed on the surface of B. subtilis RH101(Delta cotC) spores using CotC as protein carrier. Presence of LacA protein is verified by western blotting. Results of beta-galactiosidase assay show that the expressed enzyme retained its activity in condition of freezing and drying, as well as after recovery from the reaction's mixture.
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