期刊
ARCHIVES OF MICROBIOLOGY
卷 194, 期 8, 页码 719-724出版社
SPRINGER
DOI: 10.1007/s00203-012-0804-3
关键词
Porphyromonas gingivalis; HmuY; Non-iron metalloporphyrins; Heme; Biofilm; Epithelial cell invasion
类别
资金
- Department of Scientific Research, Ministry of Science and Higher Education [N401 029 32/0742, N N303 518438]
- Wroclaw Research Center EIT [POIG 01.01.02-02-003/08/00]
- European Regional Development Fund [1.1.2]
Porphyromonas gingivalis acquires heme for growth, and initiation and progression of periodontal diseases. One of its heme acquisition systems consists of the HmuR and HmuY proteins. This study analyzed the antimicrobial activity of non-iron metalloporphyrins against P. gingivalis during planktonic growth, biofilm formation, epithelial cell adhesion and invasion, and employed hmuY, hmuR and hmuY-hmuR mutants to assess the involvement of HmuY and HmuR proteins in the acquisition of metalloporphyrins. Iron(III) mesoporphyrin IX (mesoheme) and iron(III) deuteroporphyrin IX (deuteroheme) supported planktonic growth of P. gingivalis cells, biofilm accumulation, as well as survival, adhesion and invasion of HeLa cells in a way analogous to protoheme. In contrast, cobalt(III), gallium(III) and copper(II) protoporphyrin IX exhibited antimicrobial activity against P. gingivalis, and thus represent potentially useful antibacterial compounds with which to target P. gingivalis. P. gingivalis hmuY, hmuR and hmuY-hmuR mutants showed decreased growth and infection of epithelial cells in the presence of all metalloporphyrins examined. In conclusion, the HmuY protein may not be directly involved in transport of free metalloporphyrins into the bacterial cell, but it may also play a protective role against metalloporphyrin toxicity by binding an excess of these compounds.
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