期刊
ARCHIVES OF MICROBIOLOGY
卷 192, 期 6, 页码 447-459出版社
SPRINGER
DOI: 10.1007/s00203-010-0570-z
关键词
Hyperthermophile; Anaerobic; Oxidative stress; Peroxide response
类别
资金
- Norwegian Research Council
- National Institutes of Health [GM 60329]
- Department of Energy [FG05-95ER20175, FG02-08ER64690]
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM060329] Funding Source: NIH RePORTER
Pyrococcus furiosus is a shallow marine, anaerobic archaeon that grows optimally at 100A degrees C. Addition of H2O2 (0.5 mM) to a growing culture resulted in the cessation of growth with a 2-h lag before normal growth resumed. Whole genome transcriptional profiling revealed that the main response occurs within 30 min of peroxide addition, with the up-regulation of 62 open reading frames (ORFs), 36 of which are part of 10 potential operons. More than half of the up-regulated ORFs are of unknown function, while some others encode proteins that are involved potentially in sequestering iron and sulfide, in DNA repair and in generating NADPH. This response is thought to involve primarily damage repair rather than protection, since cultures exposed to sub-toxic levels of H2O2 were not more resistant to the subsequent addition of H2O2 (0.5-5.0 mM). Consequently, there is little if any induced protective response to peroxide. The organism maintains a constitutive protective mechanism involving high levels of oxidoreductase-type enzymes such as superoxide reductase, rubrerythrin, and alkyl hydroperoxide reductase. Related hyperthermophiles contain homologs of the proteins involved in the constitutive protective mechanism but these organisms were more sensitive to peroxide than P. furiosus and lack several of its peroxide-responsive ORFs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据