4.1 Article

Effects of complex organic extracts on callus growth and PLB regeneration through embryogenesis in the Doritaenopsis orchid

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JARQ-JAPAN AGRICULTURAL RESEARCH QUARTERLY
卷 37, 期 4, 页码 229-235

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JAPAN INT RESEARCH CENTER AGRICULTURAL SCIENCES
DOI: 10.6090/jarq.37.229

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potato extract; corn extract; papaya extract

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Potato extract (PE), corn extract (CE) and papaya extract (PAE) at concentrations of 25, 50 and 100 mL L-1 enhanced Callus growth on New Phalaenopsis (NP) medium containing sucrose. Among the various concentrations, 100 mL L-1 PE or PAE and 200 ml L-1 CE significantly promoted callus growth compared to the control. Regeneration of protocorm-like bodies (PLBs) from calli was optimum when NP medium (sometimes referred to as BM) was supplemented with 100 mL L (CE)-C-1, followed by PE and PAE at the same concentration. Most plantlets also regenerated from PLBs with one 100 mL L (CE)-C-1.

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