期刊
ELECTROPHORESIS
卷 24, 期 19-20, 页码 3476-3483出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/elps.200305611
关键词
capillary electrophoresis; field-amplified sample stacking; microfluidic chip; miniaturization; photoinitiated polymerization
Field-amplified sample stacking (FASS) leverages conductivity gradients between a volume of injected sample and the background buffer to increase sample concentration. A major challenge in applying FASS to on-chip assays is the initial setup of high-conductivity gradient boundaries in the region of the injected sample volume. We have designed, fabricated, and characterized a novel FASS-capillary electrophoresis (CE) chip design that uses a photoinitiated porous polymer structure to facilitate sample injection and flow control for high-gradient FASS. This polymer structure provides a region of high flow resistance that allows the electromigration of sample ions. We have demonstrated an electropherogram signal increase by a factor of 1100 in electrophoretic separations of fluorescein and Bodipy with, respectively, 2 muM and 1 muM initial concentrations.
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